The study investigated the demographic characteristics in the attitude of postgraduate students towards the use of information resources in university libraries in south-south Nigeria. The specific purpose was to examine the differences in the attitude of male and female postgraduate students towards the use of information resources, explore the disciplinary differences in the attitude of postgraduate students towards the use of information resources, in university libraries located in Edo, Delta, Bayelsa, Akwa-Ibom, Cross River, and Rivers States. Four research questions and four hypotheses were raised to guide this study. The survey method was adopted. A sample of 1,744 was drawn out of the population of 17,449 from which 1,412 was found usable for the study. The reliability of the instrument used for the survey was tested using Cronbach Alpha which yielded an index of (0.87). The data were analyzed using frequency counts, percentage, mean, standard deviation, z-test and ANOVA. The results of analysis revealed that Sex difference and disparate academic discipline influence Postgraduate students’ attitude towards the use of information resources in university libraries in South-South Nigeria. It was recommended that the libraries should be well funded in order to acquire current and relevant information materials, organize symposium on available information resources as part of orientation programme for postgraduate students. Assignments that compel postgraduate students to use library information resources should be encouraged by the academia. Finally the Reference librarians should be accessible for information resources consultation.